News
U.S. unprepared for foreign animal disease outbreak
A pork industry leader says the U.S. needs to be better prepared to handle a foreign animal disease outbreak.
On Thursday, past president of the National Pork Producers Council (NPPC) Dr. Howard Hill told a Senate Agriculture subcommittee hearing on livestock that foot-and-mouth disease (FMD) poses a serious threat to hog farmers.
“USDA and the livestock industry has been working on a plan to combat an outbreak, but the only practical way is through the use of vaccination. Unfortunately we currently don’t have the ability to produce the number of doses needed for an initial outbreak, or the capacity to produce more vaccine.”
Consistent with a Homeland Security presidential directive, Hill says an adequate FMD vaccine bank must be established.
“This would require an off-shore vendor-maintained bank that would have available anigen concentrate to produce against all 23 of the most common foot-and-mouth disease types currently circulating in the world.”
The pork industry is also calling for a vendor-managed inventory of 10 million doses, which Hill says is the estimated need during the first two weeks of an FMD outbreak.
He also told lawmakers a contract with an international vaccine manufacturer with the surge capacity of at least 40 million additional doses is necessary.
-
-
And by an “international vaccine vendor” I am assuming this would be – China?! Wonder how much money has changed hands this time?
-
CWD of deer and elk is spreading across North America and cannot be stopped.
The tse prion aka mad cow type disease is not your normal pathogen.
The TSE prion disease survives ashing to 600 degrees celsius, that’s around 1112 degrees farenheit.
You cannot cook the TSE prion disease out of meat. you can take the ash and mix it with saline and inject that ash into a mouse, and the mouse will go down with TSE.
Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel Production as well.
The TSE prion agent also survives Simulated Wastewater Treatment Processes.
IN fact, you should also know that the TSE Prion agent will survive in the environment for years, if not decades.
You can bury it and it will not go away.
The TSE agent is capable of infected your water table i.e. Detection of protease-resistant cervid prion protein in water from a CWD-endemic area.
it’s not your ordinary pathogen you can just cook it out and be done with.
that’s what’s so worrisome about Iatrogenic mode of transmission, a simple autoclave will not kill this TSE prion agent.
cwd to humans, consumption, exposure, sub-clinical, iatrogenic, what if ?
i strenuously urge you all to rethink this cutting of funds for research of the TSE Prion disease.
seems if my primitive education does not fail me, intracranial means inside the skull, and peroral means by the mouth. seems the price of tse prion poker just keeps going up…terry
Research Project: TRANSMISSION, DIFFERENTIATION, AND PATHOBIOLOGY OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES
Location: Virus and Prion Research
Title: Disease-associated prion protein detected in lymphoid tissues from pigs challenged with the agent of chronic wasting disease
Author item Moore, Sarah item Kunkle, Robert item Kondru, Naveen item Manne, Sireesha item Smith, Jodi item Kanthasamy, Anumantha item West Greenlee, M item Greenlee, Justin
Submitted to: Prion Publication Type: Abstract Only Publication Acceptance Date: 3/15/2017 Publication Date: N/A Citation: N/A Interpretive Summary:
Technical Abstract: Aims: Chronic wasting disease (CWD) is a naturally-occurring, fatal neurodegenerative disease of cervids. We previously demonstrated that disease-associated prion protein (PrPSc) can be detected in the brain and retina from pigs challenged intracranially or orally with the CWD agent. In that study, neurological signs consistent with prion disease were observed only in one pig: an intracranially challenged pig that was euthanized at 64 months post-challenge. The purpose of this study was to use an antigen-capture immunoassay (EIA) and real-time quaking-induced conversion (QuIC) to determine whether PrPSc is present in lymphoid tissues from pigs challenged with the CWD agent. Methods: At two months of age, crossbred pigs were challenged by the intracranial route (n=20), oral route (n=19), or were left unchallenged (n=9). At approximately 6 months of age, the time at which commercial pigs reach market weight, half of the pigs in each group were culled (6 month challenge groups) were allowed to incubate for up to 73 months post challenge (mpc). The retropharyngeal lymph node (RPLN) was screened for the presence of PrPSc by EIA and immunohistochemistry (IHC). The RPLN, palatine tonsil, and mesenteric lymph node (MLN) from 6-7 pigs per challenge group were also tested using EIA and QuIC. Results: PrPSc was not detected by EIA and IHC in any RPLNs. All tonsils and MLNs were negative by IHC, though the MLN from one pig in the oral <6 month group was positive by EIA. PrPSc was detected by QuIC in at least one of the lymphoid tissues examined in 5/6 pigs in the intracranial 6 months group, 5/6 pigs in the oral 6 months group. Overall, the MLN was positive in 14/19 (74%) of samples examined, the RPLN in 8/18 (44%), and the tonsil in 10/25 (40%).
Conclusions:
This study demonstrates that PrPSc accumulates in lymphoid tissues from pigs challenged intracranially or orally with the CWD agent, and can be detected as early as 4 months after challenge.
CWD-infected pigs rarely develop clinical disease and if they do, they do so after a long incubation period.
This raises the possibility that CWD-infected pigs could shed prions into their environment long before they develop clinical disease.
Furthermore, lymphoid tissues from CWD-infected pigs could present a potential source of CWD infectivity in the animal and human food chains.
https://www.ars.usda.gov/research/publications/publication/?seqNo115=337105
CONFIDENTIAL
EXPERIMENTAL PORCINE SPONGIFORM ENCEPHALOPATHY
While this clearly is a cause for concern we should not jump to the conclusion that this means that pigs will necessarily be infected by bone and meat meal fed by the oral route as is the case with cattle. …
http://web.archive.org/web/20031026000118/www.bseinquiry.gov.uk/files/yb/1990/08/23004001.pdf
we cannot rule out the possibility that unrecognised subclinical spongiform encephalopathy could be present in British pigs though there is no evidence for this: only with parenteral/implantable pharmaceuticals/devices is the theoretical risk to humans of sufficient concern to consider any action.
http://web.archive.org/web/20030822031154/www.bseinquiry.gov.uk/files/yb/1990/09/10007001.pdf
May I, at the outset, reiterate that we should avoid dissemination of papers relating to this experimental finding to prevent premature release of the information.
http://web.archive.org/web/20030822052332/www.bseinquiry.gov.uk/files/yb/1990/09/11005001.pdf
3. It is particularly important that this information is not passed outside the Department, until Ministers have decided how they wish it to be handled.
http://web.archive.org/web/20030822052438/www.bseinquiry.gov.uk/files/yb/1990/09/12002001.pdf
But it would be easier for us if pharmaceuticals/devices are not directly mentioned at all.
http://web.archive.org/web/20030518170213/www.bseinquiry.gov.uk/files/yb/1990/09/13004001.pdf
Our records show that while some use is made of porcine materials in medicinal products, the only products which would appear to be in a hypothetically ”higher risk” area are the adrenocorticotrophic hormone for which the source material comes from outside the United Kingdom, namely America China Sweden France and Germany. The products are manufactured by Ferring and Armour. A further product, ”Zenoderm Corium implant” manufactured by Ethicon, makes use of porcine skin – which is not considered to be a ”high risk” tissue, but one of its uses is described in the data sheet as ”in dural replacement”. This product is sourced from the United Kingdom…..
http://web.archive.org/web/20030822054419/www.bseinquiry.gov.uk/files/yb/1990/09/21009001.pdf
snip…see much more here ;
WEDNESDAY, APRIL 05, 2017
Disease-associated prion protein detected in lymphoid tissues from pigs challenged with the agent of chronic wasting disease
http://chronic-wasting-disease.blogspot.com/2017/04/disease-associated-prion-protein.html
SPONTANEOUS ATYPICAL BOVINE SPONGIFORM ENCEPHALOPATHY
***Moreover, sporadic disease has never been observed in breeding colonies or primate research laboratories, most notably among hundreds of animals over several decades of study at the National Institutes of Health25, and in nearly twenty older animals continuously housed in our own facility.***
-
*** Spraker suggested an interesting explanation for the occurrence of CWD. The deer pens at the Foot Hills Campus were built some 30-40 years ago by a Dr. Bob Davis. At or abut that time, allegedly, some scrapie work was conducted at this site. When deer were introduced to the pens they occupied ground that had previously been occupied by sheep.
http://collections.europarchive.org/tna/20080102193705/http://www.bseinquiry.gov.uk/files/mb/m11b/tab01.pdf
SUNDAY, APRIL 23, 2017FDA Sec. 589.1 589.2 Substances prohibited from use in animal food or feed Animal, proteins prohibited in ruminant feed current of April 1 2016
http://madcowfeed.blogspot.com/2017/04/fda-sec-5891-5892-substances-prohibited.html
Research Project: TRANSMISSION, DIFFERENTIATION, AND PATHOBIOLOGY OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIESTitle: Scrapie transmits to white-tailed deer by the oral route and has a molecular profile similar to chronic wasting disease Authors
item Greenlee, Justin item Moore, S – item Smith, Jodi – item Kunkle, Robert item West Greenlee, M –
Submitted to: American College of Veterinary Pathologists Meeting Publication Type: Abstract Only Publication Acceptance Date: August 12, 2015 Publication Date: N/A
Technical Abstract: The purpose of this work was to determine susceptibility of white-tailed deer (WTD) to the agent of sheep scrapie and to compare the resultant PrPSc to that of the original inoculum and chronic wasting disease (CWD). We inoculated WTD by a natural route of exposure (concurrent oral and intranasal (IN); n=5) with a US scrapie isolate. All scrapie-inoculated deer had evidence of PrPSc accumulation. PrPSc was detected in lymphoid tissues at preclinical time points, and deer necropsied after 28 months post-inoculation had clinical signs, spongiform encephalopathy, and widespread distribution of PrPSc in neural and lymphoid tissues. Western blotting (WB) revealed PrPSc with 2 distinct molecular profiles. WB on cerebral cortex had a profile similar to the original scrapie inoculum, whereas WB of brainstem, cerebellum, or lymph nodes revealed PrPSc with a higher profile resembling CWD. Homogenates with the 2 distinct profiles from WTD with clinical scrapie were further passaged to mice expressing cervid prion protein and intranasally to sheep and WTD. In cervidized mice, the two inocula have distinct incubation times. Sheep inoculated intranasally with WTD derived scrapie developed disease, but only after inoculation with the inoculum that had a scrapie-like profile. The WTD study is ongoing, but deer in both inoculation groups are positive for PrPSc by rectal mucosal biopsy.
In summary, this work demonstrates that WTD are susceptible to the agent of scrapie, two distinct molecular profiles of PrPSc are present in the tissues of affected deer, and inoculum of either profile readily passes to deer.
http:// http://www.ars.usda.gov/research/publications/publications.htm?SEQ_NO_115=317901
TUESDAY, MARCH 28, 2017
*** Passage of scrapie to deer results in a new phenotype upon return passage to sheep ***
http://chronic-wasting-disease.blogspot.com/2017/03/passage-of-scrapie-to-deer-results-in.html
***at present, no cervid PrP allele conferring absolute resistance to prion infection has been identified.
P-145 Estimating chronic wasting disease resistance in cervids using real time quaking- induced conversion
Nicholas J Haley1, Rachel Rielinqer2, Kristen A Davenport3, W. David Walter4, Katherine I O’Rourke5, Gordon Mitchell6, Juergen A Richt2
1 Department of Microbiology and Immunology, Midwestern University, United States; 2Department of Diagnostic Medicine and Pathobiology, Kansas State University; 3Prion Research Center; Colorado State University; 4U.S. Geological Survey, Pennsylvania Cooperative Fish and Wildlife Research Unit; 5Agricultural Research Service, United States Department of Agriculture; 6Canadian Food Inspection Agency, National and OlE Reference Laboratory for Scrapie and CWO
In mammalian species, the susceptibility to prion diseases is affected, in part, by the sequence of the host’s prion protein (PrP). In sheep, a gradation from scrapie susceptible to resistant has been established both in vivo and in vitro based on the amino acids present at PrP positions 136, 154, and 171, which has led to global breeding programs to reduce the prevalence of scrapie in domestic sheep. In cervids, resistance is commonly characterized as a delayed progression of chronic wasting disease (CWD); at present, no cervid PrP allele conferring absolute resistance to prion infection has been identified. To model the susceptibility of various naturally-occurring and hypothetical cervid PrP alleles in vitro, we compared the amplification rates and efficiency of various CWD isolates in recombinant PrPC using real time quaking-induced conversion. We hypothesized that amplification metrics of these isolates in cervid PrP substrates would correlate to in vivo susceptibility – allowing susceptibility prediction for alleles found at 10 frequency in nature, and that there would be an additive effect of multiple resistant codons in hypothetical alleles. Our studies demonstrate that in vitro amplification metrics predict in vivo susceptibility, and that alleles with multiple codons, each influencing resistance independently, do not necessarily contribute additively to resistance. Importantly, we found that the white-tailed deer 226K substrate exhibited the slowest amplification rate among those evaluated, suggesting that further investigation of this allele and its resistance in vivo are warranted to determine if absolute resistance to CWD is possible.
***at present, no cervid PrP allele conferring absolute resistance to prion infection has been identified.
PRION 2016 CONFERENCE TOKYO
-
CWD TSE PRION ROUNDUP
THURSDAY, MARCH 30, 2017
Norway CWD Skrantesjuke: VKM report supports the National Veterinary Institute perception management
http://chronic-wasting-disease.blogspot.com/2017/03/norway-cwd-skrantesjuke-vkm-report.html
SUNDAY, MAY 21, 2017
Arkansas Chronic Wasting Disease CWD TSE Prion Roundup 212 Cases Confirmed To Date
http://chronic-wasting-disease.blogspot.com/2017/05/arkansas-chronic-wasting-disease-cwd.html
SATURDAY, MAY 20, 2017Missouri CWD TSE PRION Surveillance and Monitoring
http://chronic-wasting-disease.blogspot.com/2017/05/missouri-cwd-tse-prion-surveillance-and.html
THURSDAY, MAY 18, 2017
Minnesota Four more farmed white-tailed deer test positive for Chronic Wasting Disease CWD TSE Prion
http://chronic-wasting-disease.blogspot.com/2017/05/minnesota-four-more-farmed-white-tailed.html
MONDAY, MARCH 27, 2017
Wyoming CWD Postive Mule Deer Doe Near Pinedale
http://chronic-wasting-disease.blogspot.com/2017/03/wyoming-cwd-postive-mule-deer-doe-near.html
MONDAY, MARCH 20, 2017
Wisconsin CWD TSE Prion Annual Roundup 441 positive
http://chronic-wasting-disease.blogspot.com/2017/03/wisconsin-cwd-tse-prion-annual-roundup.html
TUESDAY, MARCH 14, 2017
Iowa 12 deer test positive for chronic wasting disease from 2016-17 hunting seasons
http://chronic-wasting-disease.blogspot.com/2017/03/iowa-12-deer-test-positive-for-chronic.html
MONDAY, MARCH 13, 2017
CHRONIC WASTING DISEASE CWD TSE PRION UDATE March 13, 2017
http://chronic-wasting-disease.blogspot.com/2017/03/chronic-wasting-disease-cwd-tse-prion.html
FRIDAY, MARCH 10, 2017
Nebraska Tests confirm spread of CWD to Lancaster County
http://chronic-wasting-disease.blogspot.com/2017/03/nebraska-tests-confirm-spread-of-cwd-to.html
THURSDAY, MARCH 09, 2017
Missouri MDC REPORTS TWO CASES OF CWD IN ST. CLAIR COUNTY
http://chronic-wasting-disease.blogspot.com/2017/03/missouri-mdc-reports-two-cases-of-cwd.html
SATURDAY, MARCH 04, 2017
Maryland DNR Six Deer Test Positive for Chronic Wasting Disease
http://chronic-wasting-disease.blogspot.com/2017/03/maryland-dnr-six-deer-test-positive-for.html
WEDNESDAY, MARCH 01, 2017
South central Pennsylvania Captive Deer Tests Positive for Chronic Wasting Disease
http://chronic-wasting-disease.blogspot.com/2017/03/south-central-pennsylvania-captive-deer.html
MONDAY, MAY 15, 2017
Pennsylvania 25 more deer test positive for CWD TSE PRION in the wild
http://chronic-wasting-disease.blogspot.com/2017/05/pennsylvania-25-more-deer-test-positive.html
MONDAY, MAY 15, 2017
TEXAS New CWD TSE PRION Case Discovered at Fifth Captive Deer Breeding Facility
http://chronic-wasting-disease.blogspot.com/2017/05/texas-new-cwd-tse-prion-case-discovered.html
SUNDAY, MAY 14, 2017
85th Legislative Session 2017 AND THE TEXAS TWO STEP Chronic Wasting Disease CWD TSE Prion, and paying to play
http://chronic-wasting-disease.blogspot.com/2017/05/85th-legislative-session-2017-and-texas.html
FRIDAY, MARCH 31, 2017
TPWD UPDATE CWD TSE Prion 49 confirmed cases and unwanted firsts for Texas
http://chronic-wasting-disease.blogspot.com/2017/03/tpwd-update-cwd-tse-prion-49-confirmed.html
FRIDAY, JANUARY 27, 2017
TEXAS, Politicians, TAHC, TPWD, and the spread of CWD TSE Prion in Texas
http://chronic-wasting-disease.blogspot.com/2017/01/texas-politicians-tahc-tpwd-and-spread.html
MONDAY, MARCH 13, 2017
CHRONIC WASTING DISEASE CWD TSE PRION UDATE March 13, 2017
http://chronic-wasting-disease.blogspot.com/2017/03/chronic-wasting-disease-cwd-tse-prion.html
SATURDAY, JANUARY 14, 2017
CHRONIC WASTING DISEASE CWD TSE PRION GLOBAL UPDATE JANUARY 14, 2017
http://chronic-wasting-disease.blogspot.com/2017/01/chronic-wasting-disease-cwd-tse-prion.html
MONDAY, MARCH 8, 2010
Canine Spongiform Encephalopathy aka MAD DOG DISEASE
http://caninespongiformencephalopathy.blogspot.com/2010/03/canine-spongiform-encephalopathy-aka.html
MONDAY, APRIL 17, 2017
Wildlife advocates see wolves as ‘best natural defense’ against chronic wasting disease
NO WAY! this is an extremely stupid move, and very, very, dangerous…
http://chronic-wasting-disease.blogspot.com/2017/04/wildlife-advocates-see-wolves-as-best.html
WEDNESDAY, MAY 03, 2017
*** First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques ***
http://chronic-wasting-disease.blogspot.com/2017/05/first-evidence-of-intracranial-and.html
TUESDAY, JANUARY 17, 2017
FDA PART 589 — SUBSTANCES PROHIBITED FROM USE IN ANIMAL FOOD OR FEED VIOLATIONS OFFICIAL ACTION INDICATED OAI UPDATE 2016 to 2017 BSE TSE PRION
http://bovineprp.blogspot.com/2017/01/fda-part-589-substances-prohibited-from.html
http://bovineprp.blogspot.com/
TUESDAY, APRIL 18, 2017
*** EXTREME USA FDA PART 589 TSE PRION FEED LOOP HOLE STILL EXIST, AND PRICE OF POKER GOES UP ***
http://usdameatexport.blogspot.com/2017/04/extreme-usa-fda-part-589-tse-prion-feed.html
WEDNESDAY, MAY 17, 2017
SHIC FUNDED STUDY SUGGESTS POTENTIAL FOR PATHOGEN TRANSMISSION VIA FEED
WEDNESDAY, MAY 17, 2017
CWD, TSE, PRION, Cattle, Pigs, Sheep, and Humans aka Mad Cow Disease
http://chronic-wasting-disease.blogspot.com/2017/05/cwd-tse-prion-cattle-pigs-sheep-and.html
WEDNESDAY, MAY 17, 2017
*** Chronic Wasting Disease CWD TSE Prion aka Mad Deer Disease and the Real Estate Market Land Values ***
http://chronic-wasting-disease.blogspot.com/2017/05/chronic-wasting-disease-cwd-tse-prion.html
kind regards, terry
-
CWD TSE PRION UPDATE February 4, 2018
SATURDAY, FEBRUARY 03, 2018
Arkansas Reports 346 Positive CWD TSE Prion cases found as of January 8, 2018
http://chronic-wasting-disease.blogspot.com/2018/02/arkansas-reports-346-positive-cwd-tse.html
WEDNESDAY, JANUARY 24, 2018
TEXAS CHRONIC WASTING DISEASE CWD TSE PRION MOUNTING, JUMPS TO 79 CASES TO DATE
http://chronic-wasting-disease.blogspot.com/2018/01/texas-chronic-wasting-disease-cwd-tse.html
FRIDAY, JANUARY 26, 2018
WISCONSIN REPORTS 588 CWD TSE PRION POSITIVE CASES FOR 2017 WITH 4170 CASES CONFIRMED TO DATE
http://chronic-wasting-disease.blogspot.com/2018/01/wisconsin-reports-588-cwd-tse-prion.html
TUESDAY, JANUARY 30, 2018
Colorado Chronic Wasting Disease CWD TSE Prion 7/2015-6/2016 Results (2017?)
http://chronic-wasting-disease.blogspot.com/2018/01/colorado-chronic-wasting-disease-cwd.html
THURSDAY, JANUARY 25, 2018
Ohio Chronic Wasting Disease CWD TSE Prioin aka mad deer update 2016-2017 SEASON SUMMARY
http://chronic-wasting-disease.blogspot.com/2018/01/ohio-chronic-wasting-disease-cwd-tse.html
SATURDAY, JANUARY 20, 2018
Pennsylvania CWD TSE Prion Cases Explodes 51 deer from the 2017-18 hunting seasons have tested positive for CWD majority of samples collected still are being analyzed
http://chronic-wasting-disease.blogspot.com/2018/01/pennsylvania-cwd-tse-prion-cases.html
WEDNESDAY, JANUARY 24, 2018
Illinois Chronic Wasting Disease CWD TSE Prion cases mounting with 75 confirmed 2017 and 685 total to date
http://chronic-wasting-disease.blogspot.com/2018/01/illinois-chronic-wasting-disease-cwd.html
TUESDAY, JANUARY 23, 2018
Iowa Preliminary CWD TSE Prion Minimal Low Testing Reports 2 Confirmed With 5 Suspects To Date for 2017 Season
http://chronic-wasting-disease.blogspot.com/2018/01/iowa-preliminary-cwd-tse-prion-minimal.html
January 14, 2018
Michigan’s Chronic Wasting Disease Working Group Recommendations Report to the Natural Resources Commission Prepared December 2017 CWD Confirmed Cases holding for now at 57 cases
http://www.michigan.gov/emergingdiseases/0,4579,7-186-81018_25806-357110–,00.html
http://chronic-wasting-disease.blogspot.com/2018/01/michigans-chronic-wasting-disease.html
Michigan UPDATE, see also ;
Addressing deer disease: DNR, MSU collaborate on deer movement study in south-central Michigan
Contact: Dwayne Etter (DNR), 517-284-4725 or David Williams (MSU), 517-917-0716 Agency: Natural Resources
http://www.michigan.gov/som/0,4669,7-192-47796-458819–,00.html
January 14, 2018
Missouri MDC REPORTS 15 NEW CASES OF CWD TSE Prion in Deer
http://chronic-wasting-disease.blogspot.com/2018/01/missouri-mdc-reports-15-new-cases-of.html
MONDAY, JANUARY 29, 2018
Wyoming, Hanna, WGFD diagnosed chronic wasting disease (CWD) for the first time in Deer Hunt Area 161
http://chronic-wasting-disease.blogspot.com/2018/01/wyoming-hanna-wgfd-diagnosed-chronic.html
MONDAY, JANUARY 29, 2018
North Dakota CWD Confirmed whitetail buck and a mule deer doe 2017 deer gun season from unit 3F2
http://chronic-wasting-disease.blogspot.com/2018/01/north-dakota-cwd-confirmed-whitetail.html
2017
Subject: ***CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat
CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat
Chronic Wasting Disease (CWD)
Prevention
If CWD could spread to people, it would most likely be through eating of infected deer and elk. In a 2006-2007 CDC survey of U.S. residents, nearly 20 percent of those surveyed said they had hunted deer or elk and more than two-thirds said they had eaten venison or elk meat. However, to date, no CWD infections have been reported in people.
Hunters must consider many factors when determining whether to eat meat from deer and elk harvested from areas with CWD, including the level of risk they are willing to accept. Hunters harvesting wild deer and elk from areas with reported CWD should check state wildlife and public health guidance to see whether testing of animals is recommended or required in a given state or region. In areas where CWD is known to be present, CDC recommends that hunters strongly consider having those animals tested before eating the meat.
Tests for CWD are monitoring tools that some state wildlife officials use to look at the rates of CWD in certain animal populations. Testing may not be available in every state, and states may use these tests in different ways. A negative test result does not guarantee that an individual animal is not infected with CWD, but it does make it considerably less likely and may reduce your risk of exposure to CWD.
To be as safe as possible and decrease their potential risk of exposure to CWD, hunters should take the following steps when hunting in areas with CWD:
Do not shoot, handle or eat meat from deer and elk that look sick or are acting strangely or are found dead (road-kill). When field-dressing a deer: Wear latex or rubber gloves when dressing the animal or handling the meat. Minimize how much you handle the organs of the animal, particularly the brain or spinal cord tissues. Do not use household knives or other kitchen utensils for field dressing. Check state wildlife and public health guidance to see whether testing of animals is recommended or required. Recommendations vary by state, but information about testing is available from many state wildlife agencies. Strongly consider having the deer or elk tested for CWD before you eat the meat. If you have your deer or elk commercially processed, consider asking that your animal be processed individually to avoid mixing meat from multiple animals. If your animal tests positive for CWD, do not eat meat from that animal. The U.S. Department of Agriculture’s Animal and Plant Health Inspection Service regulates commercially farmed deer and elk. The agency operates a national CWD herd certification program. As part of the voluntary program, states and individual herd owners agree to meet requirements meant to decrease the risk of CWD in their herds. Privately owned herds that do not participate in the herd certification program may be at increased risk for CWD.
Page last reviewed: August 17, 2017 Page last updated: August 17, 2017 Content source: Centers for Disease Control and Prevention National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) Division of High-Consequence Pathogens and Pathology (DHCPP)
https://www.cdc.gov/prions/cwd/prevention.html
> However, to date, no CWD infections have been reported in people.
key word here is ‘reported’. science has shown that CWD in humans will look like sporadic CJD. SO, how can one assume that CWD has not already transmitted to humans? they can’t, and it’s as simple as that. from all recorded science to date, CWD has already transmitted to humans, and it’s being misdiagnosed as sporadic CJD. …terry
LOOKING FOR CWD IN HUMANS AS nvCJD or as an ATYPICAL CJD, LOOKING IN ALL THE WRONG PLACES $$$
*** These results would seem to suggest that CWD does indeed have zoonotic potential, at least as judged by the compatibility of CWD prions and their human PrPC target. Furthermore, extrapolation from this simple in vitro assay suggests that if zoonotic CWD occurred, it would most likely effect those of the PRNP codon 129-MM genotype and that the PrPres type would be similar to that found in the most common subtype of sCJD (MM1).***
http://www.tandfonline.com/doi/full/10.4161/pri.28124?src=recsys
http://www.tandfonline.com/doi/pdf/10.4161/pri.28124?needAccess=true
Molecular Barriers to Zoonotic Transmission of Prions
*** chronic wasting disease, there was no absolute barrier to conversion of the human prion protein.
*** Furthermore, the form of human PrPres produced in this in vitro assay when seeded with CWD, resembles that found in the most common human prion disease, namely sCJD of the MM1 subtype.
http://wwwnc.cdc.gov/eid/article/20/1/13-0858_article.htm
TUESDAY, SEPTEMBER 12, 2017
CDC Now Recommends Strongly consider having the deer or elk tested for CWD before you eat the meat
http://chronic-wasting-disease.blogspot.com/2017/09/cdc-now-recommends-strongly-consider.html
Prion 2017 Conference Abstracts CWD
2017 PRION CONFERENCE
First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques: a work in progress
Stefanie Czub1, Walter Schulz-Schaeffer2, Christiane Stahl-Hennig3, Michael Beekes4, Hermann Schaetzl5 and Dirk Motzkus6 1
University of Calgary Faculty of Veterinary Medicine/Canadian Food Inspection Agency; 2Universitatsklinikum des Saarlandes und Medizinische Fakultat der Universitat des Saarlandes; 3 Deutsches Primaten Zentrum/Goettingen; 4 Robert-Koch-Institut Berlin; 5 University of Calgary Faculty of Veterinary Medicine; 6 presently: Boehringer Ingelheim Veterinary Research Center; previously: Deutsches Primaten Zentrum/Goettingen
This is a progress report of a project which started in 2009. 21 cynomolgus macaques were challenged with characterized CWD material from white-tailed deer (WTD) or elk by intracerebral (ic), oral, and skin exposure routes. Additional blood transfusion experiments are supposed to assess the CWD contamination risk of human blood product. Challenge materials originated from symptomatic cervids for ic, skin scarification and partially per oral routes (WTD brain). Challenge material for feeding of muscle derived from preclinical WTD and from preclinical macaques for blood transfusion experiments. We have confirmed that the CWD challenge material contained at least two different CWD agents (brain material) as well as CWD prions in muscle-associated nerves.
Here we present first data on a group of animals either challenged ic with steel wires or per orally and sacrificed with incubation times ranging from 4.5 to 6.9 years at postmortem. Three animals displayed signs of mild clinical disease, including anxiety, apathy, ataxia and/or tremor. In four animals wasting was observed, two of those had confirmed diabetes. All animals have variable signs of prion neuropathology in spinal cords and brains and by supersensitive IHC, reaction was detected in spinal cord segments of all animals. Protein misfolding cyclic amplification (PMCA), real-time quaking-induced conversion (RT-QuiC) and PET-blot assays to further substantiate these findings are on the way, as well as bioassays in bank voles and transgenic mice.
At present, a total of 10 animals are sacrificed and read-outs are ongoing. Preclinical incubation of the remaining macaques covers a range from 6.4 to 7.10 years. Based on the species barrier and an incubation time of > 5 years for BSE in macaques and about 10 years for scrapie in macaques, we expected an onset of clinical disease beyond 6 years post inoculation.
PRION 2017 DECIPHERING NEURODEGENERATIVE DISORDERS
Subject: PRION 2017 CONFERENCE DECIPHERING NEURODEGENERATIVE DISORDERS VIDEO
PRION 2017 CONFERENCE DECIPHERING NEURODEGENERATIVE DISORDERS
*** PRION 2017 CONFERENCE VIDEO
https://www.youtube.com/embed/Vtt1kAVDhDQ
http://prion2017.org/programme/
TUESDAY, JUNE 13, 2017
PRION 2017 CONFERENCE ABSTRACT
First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques: a work in progress
http://chronic-wasting-disease.blogspot.com/2017/06/prion-2017-conference-abstract-first.html
TUESDAY, JULY 04, 2017
*** PRION 2017 CONFERENCE ABSTRACTS ON CHRONIC WASTING DISEASE CWD TSE PRION ***
http://chronic-wasting-disease.blogspot.com/2017/07/prion-2017-conference-abstracts-on.html
TUESDAY, JUNE 13, 2017
PRION 2017 CONFERENCE ABSTRACT Chronic Wasting Disease in European moose is associated with PrPSc features different from North American CWD
http://chronic-wasting-disease.blogspot.com/2017/06/prion-2017-conference-abstract-chronic.html
Wednesday, May 24, 2017
PRION2017 CONFERENCE VIDEO UPDATE 23 – 26 May 2017 Edinburgh UPDATE 1
http://prionprp.blogspot.com/2017/05/prion2017-conference-video-update-23-26.html
SATURDAY, JULY 29, 2017
Risk Advisory Opinion: Potential Human Health Risks from Chronic Wasting Disease CFIA, PHAC, HC (HPFB and FNIHB), INAC, Parks Canada, ECCC and AAFC
http://chronic-wasting-disease.blogspot.com/2017/07/risk-advisory-opinion-potential-human.html
***Moreover, sporadic disease has never been observed in breeding colonies or primate research laboratories, most notably among hundreds of animals over several decades of study at the National Institutes of Health25, and in nearly twenty older animals continuously housed in our own facility.***
http://www.nature.com/articles/srep11573
*** Infectious agent of sheep scrapie may persist in the environment for at least 16 years ***
Gudmundur Georgsson1, Sigurdur Sigurdarson2 and Paul Brown3
http://jgv.sgmjournals.org/content/87/12/3737.full
Back around 2000, 2001, or so, I was corresponding with officials abroad during the bse inquiry, passing info back and forth, and some officials from here inside USDA aphis FSIS et al. In fact helped me get into the USA 50 state emergency BSE conference call way back. That one was a doozy. But I always remember what “deep throat” I never knew who they were, but I never forgot;Some unofficial information from a source on the inside looking out –
Confidential!!!!
As early as 1992-3 there had been long studies conducted on small pastures containing scrapie infected sheep at the sheep research station associated with the Neuropathogenesis Unit in Edinburgh, Scotland. Whether these are documented…I don’t know. But personal recounts both heard and recorded in a daily journal indicate that leaving the pastures free and replacing the topsoil completely at least 2 feet of thickness each year for SEVEN years….and then when very clean (proven scrapie free) sheep were placed on these small pastures…. the new sheep also broke out with scrapie and passed it to offspring. I am not sure that TSE contaminated ground could ever be free of the agent!! A very frightening revelation!!!
—end personal email—end…tss
TUESDAY, JANUARY 30, 2018
Chronic Wasting Disease A Time Bomb For Agriculture?
WOW, i am shocked, this came from the PORK farm journal…nice article!
http://chronic-wasting-disease.blogspot.com/2018/01/chronic-wasting-disease-time-bomb-for.html
MONDAY, JANUARY 29, 2018
Assessment of Chronic Wasting Disease Prion Shedding in Deer Saliva with Occupancy Modeling
http://chronic-wasting-disease.blogspot.com/2018/01/assessment-of-chronic-wasting-disease.html
SATURDAY, FEBRUARY 03, 2018
Dehydration of prions on environmentally relevant surfaces protects them from inactivation by freezing and thawing
http://chronic-wasting-disease.blogspot.com/2018/02/dehydration-of-prions-on.html
Terry S. Singeltary Sr.
-
FRIDAY, MARCH 30, 2018
Docket No. APHIS-2018-0011 Chronic Wasting Disease Herd Certification Program Standards Singeltary Submission March 30, 2018
http://chronic-wasting-disease.blogspot.com/2018/03/docket-no-aphis-2018-0011-chronic.html
SATURDAY, MARCH 31, 2018
TEXAS DETECTS IT’S 101 CASE of CWD TSE PRION Breeder White-tailed Deer with no end in sight
http://chronic-wasting-disease.blogspot.com/2018/03/texas-detects-its-101-case-of-cwd-tse.html
you said a mouth full of tse prions there. you can’t imagine just how unprepared for a home grown animal disease the USDA really is. see for yourself ;
WS-02
Scrapie in swine: A diagnostic challenge
Justin J Greenlee1, Robert A Kunkle1, Jodi D Smith1, Heather W. Greenlee2
1National Animal Disease Center, US Dept. of Agriculture, Agricultural Research Service, United States; 2Iowa State University College of Veterinary Medicine
A naturally occurring prion disease has not been recognized in swine, but the agent of bovine spongiform encephalopathy does transmit to swine by experimental routes. Swine are thought to have a robust species barrier when exposed to the naturally occurring prion diseases of other species, but the susceptibility of swine to the agent of sheep scrapie has not been thoroughly tested.
Since swine can be fed rations containing ruminant derived components in the United States and many other countries, we conducted this experiment to test the susceptibility of swine to U.S. scrapie isolates by intracranial and oral inoculation. Scrapie inoculum was a pooled 10% (w/v) homogenate derived from the brains of clinically ill sheep from the 4th passage of a serial passage study of the U.S scrapie agent (No. 13-7) through susceptible sheep that were homozygous ARQ at prion protein residues 136, 154, and 171, respectively. Pigs were inoculated intracranially (n=19) with a single 0.75 ml dose or orally (n=24) with 15 ml repeated on 4 consecutive days. Necropsies were done on a subset of animals at approximately six months post inoculation (PI), at the time the pigs were expected to reach market weight. Remaining pigs were maintained and monitored for clinical signs of TSE until study termination at 80 months PI or when removed due to intercurrent disease (primarily lameness). Brain samples were examined by immunohistochemistry (IHC), western blot (WB), and enzyme-linked immunosorbent assay (ELISA). Brain tissue from a subset of pigs in each inoculation group was used for bioassay in mice expressing porcine PRNP.
At six-months PI, no evidence of scrapie infection was noted by any diagnostic method. However, at 51 months of incubation or greater, 5 animals were positive by one or more methods: IHC (n=4), WB (n=3), or ELISA (n=5). Interestingly, positive bioassay results were obtained from all inoculated groups (oral and intracranial; market weight and end of study).
Swine inoculated with the agent of scrapie by the intracranial and oral routes do not accumulate abnormal prion protein (PrPSc) to a level detectable by IHC or WB by the time they reach typical market age and weight. However, strong support for the fact that swine are potential hosts for the agent of scrapie comes from positive bioassay from both intracranially and orally inoculated pigs and multiple diagnostic methods demonstrating abnormal prion protein in intracranially inoculated pigs with long incubation times.
Curriculum Vitae
Dr. Greenlee is Research Veterinary Medical Officer in the Virus and Prion Research Unit at the National Animal Disease Center, US Department of Agriculture, Agricultural Research Service. He applies his specialty in veterinary anatomic pathology to focused research on the intra- and interspecies transmission of prion diseases in livestock and the development of antemortem diagnostic assays for prion diseases. In addition, knockout and transgenic mouse models are used to complement ongoing experiments in livestock species. Dr. Greenlee has publications in a number of topic areas including prion agent decontamination, effects of PRNP genotype on susceptibility to the agent of sheep scrapie, characterization of US scrapie strains, transmission of chronic wasting disease to cervids and cattle, features of H-BSE associated with the E211 K polymorphism, and the development of retinal assessment for antemortem screening for prion diseases in sheep and cattle. Dr. Greenlee obtained his DVM degree and completed the PhD/residency program in Veterinary Pathology at Iowa State University. He is a Diplomate of the American College of Veterinary Pathologists.
http://prion2016.org/
Saturday, April 23, 2016
SCRAPIE WS-01: Prion diseases in animals and zoonotic potential PRION 2016 TOKYO
Prion. 10:S15-S21. 2016 ISSN: 1933-6896 printl 1933-690X online
http://scrapie-usa.blogspot.com/2016/04/scrapie-ws-01-prion-diseases-in-animals.html
Animal and Plant Health Inspection Service (APHIS) Proposed Rule: Scrapie in Sheep and Goats Singeltary submission
https://www.regulations.gov/#!documentDetail;D=APHIS-2007-0127-0032
Wednesday, May 25, 2016
USDA APHIS National Scrapie TSE Prion Eradication Program April 2016 Monthly Report Prion 2016 Tokyo Update
http://scrapie-usa.blogspot.com/2016/05/usda-aphis-national-scrapie-tse-prion.html
see more here from PRION2015 Ft. Collins and more on zoonotic CWD ;
Monday, May 02, 2016
*** Zoonotic Potential of CWD Prions: An Update Prion 2016 Tokyo ***
http://chronic-wasting-disease.blogspot.com/2016/05/zoonotic-potential-of-cwd-prions-update.html
Tuesday, May 31, 2016
Priority Interim Position Paper PROTECTING THE FOOD CHAIN FROM PRIONS Perspectives
http://transmissiblespongiformencephalopathy.blogspot.com/2016/05/priority-interim-position-paper.html
Tuesday, May 31, 2016
New insights in the transfusional risk assessment of variant Creutzfeldt-Jakob Disease: Transfusional transmission of vCJD prions in the absence of detectable abnormal prion protein
Prion 2016 Tokyo
http://vcjd.blogspot.com/2016/05/new-insights-in-transfusional-risk.html
Saturday, May 28, 2016
Infection and detection of PrPCWD in soil from CWD infected farm in Korea Prion 2016 Tokyo
http://chronic-wasting-disease.blogspot.com/2016/05/infection-and-detection-of-prpcwd-in.html
Friday, May 27, 2016
Canine Prions: A New Form of Prion Disease EP-021 PRION 2016 TOKYO
http://caninespongiformencephalopathy.blogspot.com/2016/05/canine-prions-new-form-of-prion-disease.html
Saturday, May 28, 2016
TPWD gives in to Breeders again and postponed their decision regarding proposed changes to state regulations for managing CWD allowing the TSE Prion to spread further
http://chronic-wasting-disease.blogspot.com/2016/05/tpwd-gives-in-to-breeders-again-and.html
I strenuously once again urge the FDA and its industry constituents, to make it MANDATORY that all ruminant feed be banned to all ruminants, and this should include all cervids as soon as possible for the following reasons…
======
In the USA, under the Food and Drug Administrations BSE Feed Regulation (21 CFR 589.2000) most material (exceptions include milk, tallow, and gelatin) from deer and elk is prohibited for use in feed for ruminant animals. With regards to feed for non-ruminant animals, under FDA law, CWD positive deer may not be used for any animal feed or feed ingredients. For elk and deer considered at high risk for CWD, the FDA recommends that these animals do not enter the animal feed system.
***However, this recommendation is guidance and not a requirement by law.
======
31 Jan 2015 at 20:14 GMT
*** Ruminant feed ban for cervids in the United States? ***
31 Jan 2015 at 20:14 GMT
see Singeltary comment ;
http://www.plosone.org/annotation/listThread.action?root=85351
*** Singeltary reply ; Molecular, Biochemical and Genetic Characteristics of BSE in Canada Singeltary reply ;
http://www.plosone.org/annotation/listThread.action;jsessionid=635CE9094E0EA15D5362B7D7B809448C?root=7143
Monday, May 09, 2016
A comparison of classical and H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism in wild type and EK211 cattle following intracranial inoculation
http://bse-atypical.blogspot.com/2016/05/a-comparison-of-classical-and-h-type.html
*** Singeltary reply ; Molecular, Biochemical and Genetic
Characteristics of BSE in Canada Singeltary reply ;
http://www.plosone.org/annotation/listThread.action;jsessionid=63
*** It also suggests a similar cause or source for atypical BSE in these countries. ***
Discussion: The C, L and H type BSE cases in Canada exhibit molecular characteristics similar to those described for classical and atypical BSE cases from Europe and Japan.
*** This supports the theory that the importation of BSE contaminated feedstuff is the source of C-type BSE in Canada.
*** It also suggests a similar cause or source for atypical BSE in these countries. ***
see page 17 6 of 201 pages…tss
http://www.neuroprion.org/resources/pdf_docs/conferences/prion2009/prion2009_bookofabstracts.pdf
Docket No. FDA-2003-D-0432 (formerly 03D-0186) Use of Material from Deer and Elk in Animal Feed Singeltary Submission
https://www.regulations.gov/#!documentDetail;D=FDA-2003-D-0432-0011
Freas, William
From: Terry S. Singeltary Sr. [[email protected]]
Sent: Monday, January 08,2001 3:03 PM
TO: [email protected]
Subject: CJD/BSE (aka madcow) Human/Animal TSE’s–U.S.–Submission To Scientific Advisors and Consultants Staff January 2001 Meeting (short version)
CJD/BSE (aka madcow) Human/Animal TSE’s–U.S.–Submission To Scientific Advisors and Consultants Staff January 2001 Meeting (short version)
Greetings again Dr. Freas and Committee Members,
I wish to submit the following information to the Scientific Advisors and Consultants Staff 2001 Advisory Committee (short version).
http://www.fda.gov/ohrms/dockets/ac/01/slides/3681s2_09.pdf